恒遠産品文獻:小鼠IL-17A,IFN-γ,IL-4等試劑盒引用文獻

【文獻标題】Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto’s thyroiditis and Systemic lupus erythematosus

【作者】BING TAN，YUAN，JINYING LI，et.al

【作者單位】浙江大學（Zhejiang University）

【文獻中引用産品】

小鼠白介素17A（IL-17A）ELISA試(shì)劑(jì)盒

小鼠γ幹(gàn)擾素（IFN-γ）ELISA試劑(jì)盒

小鼠白介素4（IL-4）ELISA試(shì)劑(jì)盒

小鼠白介素10（IL-10）ELISA試(shì)劑(jì)盒

小鼠轉化生長(zhǎng)因子β（TGF-β） ELISA試劑(jì)盒

【關鍵詞】cell therapy, experimental autoimmune thyroiditis, human amniotic epithelial cells, Hashimoto’s thyroiditis，immunomodulatory, Systemic lupus erythematosus

【DOI】doi.org/10.1016/j.jcyt.2018.04.001

【影響因子(IF)】3.84

【出版期刊】《International Society》

【産品原文引用】

Serological assays

Sera cytokine levels of IL-17A, IFN-γ, IL-4, IL-10 and TGF-β of EAT and SLE mice were measured by ELISA. All ELISA kits were from Hengyuan Biological Technologies (Shanghai, China). Specific experimental steps were performed according to the manufacturer’s instructions.

Cytokine production in vitro

Splenocytes of EAT mice at the peak of disease (35 day) were stimulated with pTg in the presence or absence of hAECs. Cell-free culture supernatants were collected after 48 h by centrifugation. Cytokine levels of IL-17A, IFN-γ,IL-4, IL-10 and TGF-β were measured by ELISA(Hengyuan BiologicalTechnologies, Shanghai, China).

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